2.43
1,65
1,98
0,73
1,88
1,81
2.43
2.2 Masa molekular erlatiboaren banaketaren kalibrazio-kurban erabilitako substantzia estandarrak: intsulina, mikopeptidoak, glizina-glizina-tirosina-arginina, glizina-glizina-glizina
3 Tresna eta ekipamendua
23.2
21.4
22.2
16.1
22.3
20,8
23,9
27,5
Oro har, Sustarren produktuen aminoazidoen proportzioa Zinproren produktuena baino handiagoa da.
8. zatia Erabileraren ondorioak
Oligoelementuen iturri desberdinek errun-aldiaren amaieran oilo erruleen ekoizpen-errendimenduan eta arrautzen kalitatean duten eragina
Ekoizpen Prozesua
Kelazio-teknologia zuzendua
Zizailadura emultsio teknologia
Presiozko ihinztadura eta lehortze teknologia
Hozte eta deshezetze teknologia
Ingurumen-kontroleko teknologia aurreratua
A eranskina: Peptidoen masa molekular erlatiboaren banaketa zehazteko metodoak
Arauaren onarpena: GB/T 22492-2008
1. Probaren printzipioa:
Errendimendu handiko gel-iragazketa kromatografia bidez zehaztu zen. Hau da, betegarri porotsua fase geldikor gisa erabiliz, laginaren osagaien masa molekular erlatiboaren tamainaren aldea oinarritzat hartuta, 220nm-ko ultramoreen xurgapen-uhin-luzera duen lotura peptidikoan detektatua, gel-iragazketa kromatografia bidez masa molekular erlatiboaren banaketa zehazteko datuak prozesatzeko software espezifikoa erabiliz (hau da, GPC softwarea), kromatogramak eta haien datuak prozesatu ziren, soja-peptidoaren masa molekular erlatiboaren tamaina eta banaketa-tartea lortzeko kalkulatuz.
2. Erreaktiboak
Esperimentu-urak GB/T6682 arauan bigarren mailako uraren zehaztapena bete behar du, eta erreaktiboen erabilera, xedapen bereziak izan ezik, analitikoki purua da.
2.1 Erreaktiboen artean daude azetonitriloa (kromatografikoki purua), azido trifluoroazetikoa (kromatografikoki purua),
2.2 Masa molekular erlatiboaren banaketaren kalibrazio-kurban erabilitako substantzia estandarrak: intsulina, mikopeptidoak, glizina-glizina-tirosina-arginina, glizina-glizina-glizina
3 Tresna eta ekipamendua
3.1 Errendimendu Handiko Likido Kromatografoa (HPLC): kromatografia-estazio edo integratzaile bat, UV detektagailu batekin eta GPC datuak prozesatzeko softwarearekin.
3.2 Fase mugikorreko hutsean iragazteko eta gasifikatzeko unitatea.
3.3 Balantza elektronikoa: 0,000 1g-ko balio graduatua.
4 funtzionamendu-urrats
4.1 Baldintza kromatografikoak eta sistemaren egokitzapen esperimentuak (erreferentzia baldintzak)
- 4.1.1 Kromatografia-zutabea: TSKgelG2000swxl300 mm×7,8 mm (barne-diametroa) edo proteinak eta peptidoak zehazteko egokiak diren antzeko errendimendua duten mota bereko beste gel-zutabe batzuk.
- 4.1.2 Fase mugikorra: Azetonitriloa + ura + azido trifluoroazetikoa = 20 + 80 + 0,1.
- 4.1.3 Detekzio-uhin-luzera: 220 nm.
- 4.1.4 Emari-tasa: 0,5 mL/min.
- 4.1.5 Detekzio-denbora: 30 min.
- 4.1.6 Laginaren injekzio-bolumena: 20 μL.
- 4.1.7 Zutabearen tenperatura: giro-tenperatura.
- 4.1.8 Kromatografia-sistemak detekzio-eskakizunak betetzeko, aipatutako kromatografia-baldintzetan, gel kromatografikoko zutabearen eraginkortasuna, hau da, plaken kopuru teorikoa (N), ez zela 10000 baino txikiagoa izango zehaztu zen, tripeptido estandarraren (Glizina-Glizina-Glizina) gailurretan oinarrituta kalkulatuta.
- 4.2 Masa molekular erlatiboen kurba estandarren ekoizpena
- Goian aipatutako peptidoen estandar-disoluzio desberdinak, 1 mg/mL-ko masa-kontzentrazioa zutenak, fase mugikorren parekatzearen bidez prestatu ziren, proportzio jakin batean nahastu eta, ondoren, 0,2 μm~0,5 μm-ko poro-tamaina zuen fase organikoko mintz batetik iragazi eta laginan injektatu ziren, eta ondoren, estandarren kromatogramak lortu ziren. Masa molekular erlatiboaren kalibrazio-kurbak eta haien ekuazioak masa molekular erlatiboaren logaritmoa atxikipen-denboraren arabera irudikatuz edo erregresio linealaren bidez lortu ziren.
4.3 Laginaren tratamendua
Zehaztasunez pisatu 10 mg lagin 10 ml-ko matraze bolumen batean, gehitu fase mugikor pixka bat, astindu ultrasoinuekin 10 minutuz, lagina guztiz disolbatu eta nahastu arte, diluitu fase mugikorrarekin eskalara iritsi arte, eta ondoren iragazi 0,2 μm ~ 0,5 μm-ko poro-tamaina duen fase organikoko mintz batetik, eta iragazkia A.4.1 ataleko baldintza kromatografikoen arabera aztertu.
- 5. Masa molekular erlatiboaren banaketaren kalkulua
- 4.3an prestatutako lagin-disoluzioa 4.1eko baldintza kromatografikoetan aztertu ondoren, laginaren masa molekular erlatiboa eta bere banaketa-tartea lor daitezke laginaren datu kromatografikoak 4.2 kalibrazio-kurban GPC datuak prozesatzeko softwarea erabiliz ordezkatuz. Peptido desberdinen masa molekular erlatiboen banaketa gailurraren azaleraren normalizazio-metodoaren bidez kalkula daiteke, formula honen arabera: X=A/A total×100
- Formulan: X - Laginaren peptido osoan masa molekularreko peptido erlatibo baten masa-frakzioa, %;
- A - Peptido baten masa molekular erlatiboaren gailurraren azalera;
- A guztira - peptido masa molekular erlatibo bakoitzaren gailur-azaleren batura, hamartar batekin kalkulatuta.
- 6 Errepikagarritasuna
- Errepikagarritasun-baldintzetan lortutako bi determinazio independenteen arteko alde absolutua ez da bi determinazioen batez besteko aritmetikoaren % 15 baino handiagoa izango.
- B eranskina: Aminoazido libreak zehazteko metodoak
- Arauaren onarpena: Q/320205 KAVN05-2016
- 1.2 Erreaktiboak eta materialak
- Azido azetiko glaziarra: analitikoki purua
- Azido perklorikoa: 0,0500 mol/L
- Adierazlea: % 0,1eko kristal bioleta adierazlea (azido azetiko glaziarra)
- 2. Aminoazido askeen zehaztapena
Laginak 80 °C-tan ordubetez lehortu ziren.
Jarri lagina ontzi lehor batean giro-tenperaturara modu naturalean hoz dadin edo erabilgarri den tenperaturara hoz dadin.Pisatu gutxi gorabehera 0,1 g lagin (0,001 g-ko zehaztasuna) 250 ml-ko matraze koniko lehor batean.Jarraitu azkar hurrengo urratsera laginak inguruko hezetasuna xurgatzea saihesteko.Gehitu 25 mL azido azetiko glaziar eta nahastu ondo, gehienez 5 minutuz.Gehitu 2 tanta kristal bioleta adierazleTitratu azido perklorikoaren 0,0500 mol / L (±0,001) titrazio-disoluzio estandarrarekin, disoluzioa moretik amaierako puntura aldatu arte.
Idatzi kontsumitutako disoluzio estandarraren bolumena.
- Egin proba hutsa aldi berean.
- 3. Kalkulua eta emaitzak
- Erreaktiboko aminoazido askearen X edukia masa-frakzio gisa adierazten da (%) eta formula honen arabera kalkulatzen da: X = C × (V1-V0) × 0,1445/M × % 100, formula honetan:
- C - Azido perkloriko estandarraren disoluzioaren kontzentrazioa litroko moletan (mol/L)
- V1 - Azido perkloriko estandarraren disoluzioarekin laginen titraziorako erabilitako bolumena, mililitrotan (mL).
- Vo - Azido perklorikoko disoluzio estandarrarekin egindako titrazio-hutserako erabilitako bolumena, mililitrotan (mL);
M - Laginaren masa, gramoetan (g).
| 0,1445: Azido perkloriko estandarraren 1,00 mL-ko disoluzio baliokidea den aminoazidoen batez besteko masa [c (HClO4) = 1,000 mol / L]. | 4.2.3 Zerio sulfatoaren titrazio-soluzio estandarra: c kontzentrazioa [Ce (SO4) 2] = 0,1 mol/L, GB/T601 araudiaren arabera prestatua. | |
| Arauen onarpena: Q/70920556 71-2024 | 1. Determinazio-printzipioa (Fe adibide gisa) | Aminoazidoen burdin konplexuek oso disolbagarritasun txikia dute etanol anhidroan eta metal ioi askeak etanol anhidroan disolbagarriak dira, bien arteko disolbagarritasun aldea etanol anhidroan erabili zen aminoazidoen burdin konplexuen kelazio-tasa zehazteko. |
| Formulan: V1 - proba-soluzioaren titraziorako kontsumitutako zerio sulfatoaren disoluzio estandarraren bolumena, mL; | Etanol anhidroa; gainerakoa GB/T 27983-2011ko 4.5.2 klausularen berdina da. | 3. Analisiaren urratsak |
| Egin bi saiakuntza paraleloan. Pisatu 103±2℃-tan lehortutako laginaren 0,1g ordubetez, 0,0001g-ko zehaztasunarekin, gehitu 100mL etanol anhidro disolbatzeko, iragazi, garbitu iragazkiaren hondakina 100mL etanol anhidroarekin gutxienez hiru aldiz, eta ondoren eraman hondakina 250mL-ko matraze koniko batera, gehitu 10mL azido sulfuriko disoluzio GB/T27983-2011ko 4.5.3 klausularen arabera, eta ondoren jarraitu urrats hauek GB/T27983-2011ko 4.5.3 klausularen arabera, "Berotu disolbatzeko eta gero hozten utzi". Egin proba hutsa aldi berean. | 4. Burdin eduki totalaren zehaztapena | 4.1 Zehaztapen-printzipioa GB/T 21996-2008ko 4.4.1 klausularen berdina da. |
4.2. Erreaktiboak eta disoluzioak
| 4.2.1 Azido nahasia: Gehitu 150 ml azido sulfuriko eta 150 ml azido fosforiko 700 ml uretara eta nahastu ondo. | 4.2.2 Sodio difenilamina sulfonatoaren adierazle-soluzioa: 5 g/L, GB/T603 araudiaren arabera prestatua. | 4.2.3 Zerio sulfatoaren titrazio-soluzio estandarra: c kontzentrazioa [Ce (SO4) 2] = 0,1 mol/L, GB/T601 araudiaren arabera prestatua. | |
| 4.3 Analisiaren urratsak | Egin bi saiakuntza paraleloan. Pisatu 0,1 g lagin, 020001 g-ko zehaztasunarekin, jarri 250 ml-ko matraze koniko batean, gehitu 10 ml azido nahasi, disolbatu ondoren, gehitu 30 ml ur eta 4 tanta sodio dianilina sulfonato adierazle disoluzio, eta ondoren jarraitu urrats hauek GB/T21996-2008 agiriaren 4.4.2 klausularen arabera. Egin proba hutsa aldi berean. | 4.4 Emaitzen irudikapena | Aminoazidoen burdin konplexuen X1 burdin eduki osoa burdinaren masa-frakzioari dagokionez, %-tan adierazitako balioa, (1) formularen arabera kalkulatu zen: |
| X1=(V-V0)×C×M×10-3×100 | V0 - zerio sulfatoaren disoluzio estandarra disoluzio hutsaren titraziorako kontsumitua, mL; | V0 - zerio sulfatoaren disoluzio estandarra disoluzio hutsaren titraziorako kontsumitua, mL; | C - Zerio sulfatoaren disoluzio estandarraren kontzentrazio erreala, mol/L5. Kelatoetan burdin edukiaren kalkuluaKelatoan dagoen X2 burdin edukia, burdinaren masa-frakzioari dagokionez, %-tan adierazitako balioa, formula honen arabera kalkulatu zen: x2 = ((V1-V2) × C × 0,05585)/m1 × 100 |
| Formulan: V1 - proba-soluzioaren titraziorako kontsumitutako zerio sulfatoaren disoluzio estandarraren bolumena, mL; | V2 - zerio sulfatoaren disoluzio estandarra disoluzio hutsaren titraziorako kontsumituta, mL;nom1-Laginaren masa, g. Hartu paraleloan egindako determinazio-emaitzen batez besteko aritmetikoa determinazio-emaitza gisa, eta paraleloan egindako determinazio-emaitzen arteko aldea ez da % 0,3 baino handiagoa izango. | 0,05585 - burdin ferrosoaren masa, gramotan adierazita, C[Ce(SO4)2.4H20] = 1.000 mol/L zerio sulfatoaren 1.00 mL-ren baliokidea.nom1-Laginaren masa, g. Hartu paraleloan egindako determinazio-emaitzen batez besteko aritmetikoa determinazio-emaitza gisa, eta paraleloan egindako determinazio-emaitzen arteko aldea ez da % 0,3 baino handiagoa izango. | 6. Kelazio-tasaren kalkuluaKelazio-tasa X3, %-tan adierazitako balioa, X3 = X2/X1 × 100C eranskina: Zinproren kelazio-tasa zehazteko metodoak |
Arauaren onarpena: Q/320205 KAVNO7-2016
1. Erreaktiboak eta materialak
a) Azido azetiko glaziarra: analitikoki purua; b) Azido perklorikoa: 0,0500 mol/L; c) Adierazlea: % 0,1eko kristal bioleta adierazlea (azido azetiko glaziarra)
2. Aminoazido askeen zehaztapena
2.1 Laginak 80 °C-tan ordubetez lehortu ziren.
2.2 Jarri lagina ontzi lehor batean giro-tenperaturara modu naturalean hozteko edo erabilgarri den tenperaturara hozteko.
2.3 Pisatu gutxi gorabehera 0,1 g lagin (0,001 g-ko zehaztasuna) 250 mL-ko matraze koniko lehor batean.
2.4 Jarraitu azkar hurrengo urratsera laginak inguruko hezetasuna xurga ez dezan.
2.5 Gehitu 25 ml azido azetiko glaziar eta nahastu ondo, gehienez 5 minutuz.
2.6 Gehitu kristal bioleta adierazlearen 2 tanta.
2.7 Titratu azido perklorikoaren 0,0500 mol/L (±0,001) titrazio-disoluzio estandarrarekin, disoluzioa moretik berdera 15 segundoz aldatu arte, amaierako puntuan kolorea aldatu gabe.
2.8 Kontsumitutako disoluzio estandarraren bolumena erregistratu.
2.9 Egin proba hutsa aldi berean.
- 3. Kalkulua eta emaitzak
- Katalana
- Physicochemical parameters
V1 - Azido perkloriko estandarraren disoluzioarekin laginen titraziorako erabilitako bolumena, mililitrotan (mL).
Vo - Azido perklorikoko disoluzio estandarrarekin egindako titrazio-hutserako erabilitako bolumena, mililitrotan (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Helbidea: Qingpu errepidea, 147. zk., Shouan herria, Pujiang konderria, Chengdu hiria, Sichuan probintzia, Txina
Telefonoa: 86-18880477902
Produktuak
Mineral ez-organiko arrastoak
- Mineral organiko arrastoak
- Swahilia
- Zerbitzu pertsonalizatua
- Esteka azkarrak
Enpresaren profila
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujaratiarra | Egin klik kontsulta egiteko | © Copyright - 2010-2025 : Eskubide guztiak erreserbatuta. | Gunearen mapa BILAKETA NAGUSIAK Telefonoa |
| Telefonoa | 86-18880477902 | Javanera | Posta elektronikoa |
| 8618880477902 | Txinera | Frantsesa | |
| Bird | Txinera | Frantsesa | Alemana Gaztelania |
| Aquatic animals | Japoniera | Koreera | Arabiera Greziera |
| Turkiarra | Italiera | ||
| Ruminant animal g/head day | January 0.75 | Indonesiera Afrikaans Suediera |
Poloniera
- Euskera
- Katalana
- Physicochemical parameters
Hindia
Laos
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgariera
- Cebuera
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kroaziera
Nederlandera
| Application object | Urduera Vietnamdarra | Content in full-value feed (mg/kg) | Efficacy |
| Gujaratiarra | Haitiarra | Hausa | Kinyarwanda Hmong Hungariarra |
| Piglets and fattening pigs | Igbo | Javanera | Kannada Khmerra Kurdua |
| Kirgiz | Latin | ||
| Bird | 300～400 | 45～60 | Mazedoniarra Malaysiarra Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norvegiera
- Paxtuera
- Appearance: brownish-yellow granules
- Physicochemical parameters
serbierarra
Sesothoera
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahilia
Tajik
Tamil
Telugu
Thailandiarra
| Application object | Urduera Vietnamdarra | Content in full-value feed (mg/kg) | Efficacy |
| Yiddish | Yoruba | Zuluera | Kinyarwanda Oriya Turkmeniera |
| Uigur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
